Effects of beta-cyclodextrin-dextran polymer on stability properties of trypsin |
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Authors: | Fernández Michael Villalonga Maria L Caballero Julio Fragoso Alex Cao Roberto Villalonga Reynaldo |
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Affiliation: | Enzyme Technology Group, Center for Biotechnological Studies, University of Matanzas, Matanzas, C.P. 44740, Cuba. |
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Abstract: | Dextran modified with the mono-6-pentylene-diamino-6-deoxy-beta-cyclodextrin derivative was evaluated as a thermoprotectant additive for trypsin. The optimum temperature for trypsin activity was increased by 7 degrees C in the presence of this polymer. The enzyme thermostability was increased from 48.5 to 64 degrees C over 10 min of incubation, and the activation free energy of thermoinactivation at 50 degrees C was increased by 4.1 kJ/mol in the presence of the additive. Trypsin was 6-fold more resistant to autolytic inactivation at alkaline pH in the presence of the polymer. |
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Keywords: | β‐cyclodextrin dextran enzyme stability trypsin |
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