Rotation of Escherichia coli F(1)-ATPase. |
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| Authors: | H Noji K H?sler W Junge K Kinosita M Yoshida S Engelbrecht |
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| Institution: | CREST team13 (Core Research for Evolutional Science and Technology) "Genetic Programming" Team 13, Teikyo University Biotechnology Research Center 3F, Nogawa 907, Kawasaki, Miyamae-ku, 216-0001, Japan. |
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| Abstract: | By applying the same method used for F(1)-ATPase (TF(1)) from thermophilic Bacillus PS3 (Noji, H., Yasuda, R., Yoshida, M., and Kinosita, K., Jr. (1997) Nature 386, 299-302), we observed ATP-driven rotation of a fluorescent actin filament attached to the gamma subunit in Escherichia coli F(1)-ATPase. The torque value and the direction of the rotation were the same as those observed for TF(1). F(1)-ATPases seem to share common properties of rotation irrespective of the sources. |
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