Effect of melatonin on cholestatic oxidative stress under constant light exposure |
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Authors: | Cruz Adolfo Padillo Francisco J Granados Jose Túnez Isaac Muñoz M Carmen Briceño Javier Pera-Madrazo Carlos Montilla Pedro |
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Affiliation: | Department of General Surgery, Reina Sofía University Hospital, Córdoba, Spain. |
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Abstract: | This study was designed to evaluate the effect of melatonin on cholestatic oxidative stress under constant light exposure. Cholestasis was induced by double ligature and section of the extra-hepatic bile duct. Melatonin was injected i.p.(1000 microg kg(-1) day(-1)). Malondialdehyde, reduced glutathione, catalase, superoxide dismutase, glutathione reductase, peroxidase and transferase were determined in liver. After bile-duct obstruction and under constant light exposure, an increase in malondialdehyde (p < 0.05) and a slight decrease in reduced glutathione were seen. Enzyme activity, with the exception of glutathione reductase, had significantly diminished. After melatonin administration, malondialdehyde fell (p < 0.001), whereas there was an increase in reduced glutathione (p < 0.0001) compared with untreated controls. Constant light exposure was associated with an increase in hepatic oxidative stress. Treatment with melatonin decreased lipid peroxide synthesis, and permitted a recovery of both reduced glutathione and scavenger enzyme activity. |
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Keywords: | obstructive jaundice oxidative stress light melatonin |
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