火力楠种胚组培快繁研究

为探索火力楠组培快繁技术体系,以其优良家系种子为材料,从外植体选材与消毒、基本培养基、PVP浓度、生根促进剂类型及浓度等方面开展试验研究。结果表明:①应用0.1%的升汞溶液消毒胚6~9 min,无菌萌发率达60%~65%。②LY培养基适宜大部分胚系的增殖生长,35 d转接1次,增殖倍数高达3.50,高3.0 cm以上芽苗比率达50%以上。③应用4~8 g·L^-1浓度的PVP能够显著降低培养基褐化程度,促进芽苗抽高生长。④各胚系之间生根难易程度差异极显著,其生根率为0~93.6%,两种生根促进剂的最佳浓度均为8.5 mg·L^-1,ABT2号生根粉的生根诱导效果极显著(P<0.01)优于IBA。⑤生根瓶苗移植30 d后成活率达90%以上,未生根瓶苗移植60 d后成活率达80%以上。本研究为火力楠优良无性系的组培快繁研究及产业化生产提供理论与技术基础。

Tissue Culture via Seed Embryo for Michelia macclurei

In order to explore tissue culture and micro-propagation technical system of Michelia macclurei,the seeds from its elite families were used to carry out experimental studies at aspects of explants selection and disinfection,basic medium,PVP concentration,type and concentration of rooting promoter.The results showed that:①The embryos were well sterilized with 0.1%mercury solution for 6-9 min,and the aseptic germination rate could reach 60%-65%;②LY medium was the best basic medium,and suitable for the proliferation and growth of most embryo lines;③Application of PVP with concentration of 4-8 g·L^-1 could significantly reduce browning degree of medium and promote growth of the shoots;④There were significant differences in rooting ability and rooting rate among tested embryo lines,the optimal concentration of both rooting promoters was 8.5 mg·L^-1,and the rooting induction effect of ABT2 root powder was significantly better than that of IBA(P<0.01);⑤The survival rate reached above 90%for rooting plantlets in 30 d,and more than 80%for plantlets with no root in 60 d after transplanted.This study provides a theoretical and technical basis for study on tissue culture and micro-propagation of M.macclurei clones and their industrial production.