萘乙酸对阳春砂不同插穗扦插繁殖效果的影响

以阳春砂根状茎茎尖、根状茎、直立茎芦头3种不同材料为插穗,研究不同浓度的ɑ-萘乙酸(NAA)对扦插效果的影响,以筛选出最佳的NAA处理浓度,寻找除分株繁殖外的高效营养繁殖方法。结果表明,未经NAA处理的根状茎茎尖、根状茎和直立茎芦头3种插穗的出芽率和生根率比较低,出芽率依次为10.0%、3.3%和23.3%;生根率依次为23.3%、10.0%和3.3%。而200 mg·L-1的NAA处理可以显著提高根状茎茎尖、根状茎和直立茎芦头3种插穗的存活率、出芽率和生根率;与对照比较,3种插穗的存活率依次提高17.7%、23.4%和26.7%;出芽率依次提高13.3%、50.3%和53.3%;生根率依次提高43.3%、26.7%和53.4%。200 mg·L-1的NAA处理可以显著提高3种插穗再生根和再生芽的生长指标。根状茎茎尖再生根的数量、长度、直径分别比对照提高56.7%、82.6%和29.2%;根状茎的再生根长度显著比对照长203.5%;直立茎芦头的再生芽的长度和直径比对照提高25.2%和35.2%。NAA处理后的根状茎茎尖插穗的芽再生方式有两种,一种是原来的茎尖继续生长,另一种是从插穗的节上分化出新芽。阳春砂根状茎茎尖、根状茎和直立茎芦头3种插穗经200 mg·L-1NAA处理后可以显著提高成活率,这为阳春砂种苗生产建立了分株繁殖以外的另一种高繁殖系数的营养繁殖方法。

Effects of α-naphthalene Acetic Acid on Cutting Propagation Efficiency of Amomum villosum

To obtain a high-efficiency method of vegetative propagation for Amomum villosum,we studied theeffects of different concentrations of α-naphthalene acetic acid(NAA)added on three kinds of cuttings of tip ofrhizome(TR),rhizome and erect stem basal(ESB). The results showed the budding rate and rooting rate ofcuttings of TR,rhizome and ESB which were not treated by NAA were at a low level respectively. The buddingrates of the three cuttings were 10.0%,3.3% and 23.3% respectively,and the rooting rates were 23.3%,10.0%and 3.3%,respectively. Compared with controls,living rates,budding rates and rooting rates of three kinds ofcuttings were increased dramatically. In sequence of TR,rhizome and ESB,living rates of three kinds ofcuttings were increased by 17.7%,23.4% and 26.7% respectively,budding rates were increased by 3.3%,50.3% and 53.3% respectively,and rooting rates were increased by 43.3%,26.7% and 53.4% respectively. Thegrowth indexes of roots regenerated from three kinds of cuttings which were treated by 200 mg·L-1 NAA wereimproved obviously. Comparing with controls,the number,length and diameter of roots regenerated from TRwere increased by 56.7%,82.6% and 29.2% respectively,the length of roots regenerated from rhizome wasincreased by 203.5%,and the length and diameter of buds regenerated from ESB were increased by 25.2% and35.2% respectively. There were two ways that buds regenerated from the cuttings of TR treated by NAA. Onewas the original bud of tip recovered growing,but the other was the new bud regenerated from cutting nodes.NAA affected the cuttings of tip of rhizome,rhizome and erect stem basal in survival rate. Survival rates of threecuttings treated by 200 mg·L-1 NAA increased dramatically comparing with control. Besides divisionpropagation,a new high-efficiency method for vegetative propagation of A.villosum was set up.