2个北美冬青新品种组培快繁高效再生技术体系的建立

以北美冬青(Ilex verticillata L.)“Red Sprite”和“Winter Gold”2个品种的幼嫩枝条为外植体,建立了2个新品种的高效组培再生技术体系,为北美冬青新品种苗木的推广提供技术支持。结果表明:腋芽诱导的最佳培养基为MS+1.0 mg·L^-16-苄氨基腺嘌呤(6-BA)+0.2 mg·L^-1萘乙酸(NAA),2个品种的诱导率都达到100%;最佳丛生芽增殖培养基为MS+0.1mg·L^-1 NAA+0.5mg·L^-16-BA+0.5 mg·L^-1氯吡苯脲(CPPU),其中品种“Red Sprite”的增殖系数达5.7,“Winter Gold”的增殖系数为4.5;最佳壮苗培养基为“Red Sprite”为MS+0.1 mg·L^-1NAA+0.5 mg·L^-16-BA+1.0 mg·L^-1噻苯隆(thifenolone,TDZ)+1.0 mg·L^-1赤霉素(GA),培养25天时苗高达6.8 cm,“Winter Gold”的为MS+0.1 mg·L^-1 NAA+0.5mg·L^-16-BA+0.05mg·L^-1 TDZ+1.0 mg·L^-1GA,25天时苗高达4.4 cm;最佳生根诱导培养基为1/2MS+0.4 mg·L^-1 NAA+0.4 mg·L^-1吲哚丁酸(IBA)+1.0 g·L^-1活性炭(AC),“Red Sprite”品种生根率达95.0%,“Winter Gold”的生根率达97.0%;最佳移栽基质为泥炭土∶珍珠岩=3∶2,“Red Sprite”成活率为95.7%,“Winter Gold”的96.8%。

Establishing an Efficient Regeneration Protocol in vitro for Rapid Micro-propagation of Two New Varieties of Ilex verticillata L.

An efficient and rapid micro-propagation system using tissue culture for two new varieties“Red Sprite”and“Winter Gold”of Ilex verticillata L.was established with the young shoots as explants,and an optimum method produced large numbers of genetically uniform seedlings and maintained its excellent character for I.verticillata L was offered.The optimal medium for axillary bud induction was MS+1.0 mg·L^-16-BA+0.2 mg·L^-1 NAA,and the induction rate of both varieties were 100%respectively.The preferred medium for cluster bud proliferation was MS+0.1 mg·L^-1 NAA+0.5 mg·L^-16-BA+0.5 mg·L^-1 CPPU.The highest proliferation coefficient was 5.7 for“Red Sprite”,and 4.5 for“Winter Gold”for 25 d culturing respectively.The optimal seedling medium for“Red Sprite”was MS+0.1 mg·L^-1 NAA+0.5 mg·L^-16-BA+1.0 mg·L^-1 TDZ+1.0 mg·L^-1 GA,and that of“Winter Gold”was MS+0.1 mg·L^-1 NAA+0.5 mg·L^-16-BA+0.05 mg·L^-1 TDZ+1.0 mg·L^-1 GA,the seedling height was up to 6.80 and 4.38 cm respectively.The optimal rooting induction medium was 1/2MS+0.4 mg·L^-1 NAA+0.4 mg·L^-1 IBA+1.0 mg?L^-1 activated carbon,the rooting rate of“Red Sprite”and“Winter Gold”were 95.0%,or 97.0%respectively.The best transplanting substrates were peat∶perlite=3∶2,with a survival rate of 95.7%for“Red Sprite”and 96.8%for“Winter Gold”respectively.