Southern elephant seal (Mirounga leonina)
II. Studies of milk protein fractions by gel electrophoresis |
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| Authors: | P A Ronayne de Ferrer R A Gonzalez Colaso M E I Marquez A R Carlini D F Vergani G A Daneri |
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| Institution: | (1) Department of Nutrition and Food Science, School of Pharmacy and Biochemistry, Junin 956, 2 P., University of Buenos Aires, (1113) Buenos Aires, Argentina, AR;(2) Department of Biological Sciences, Argentine Antarctic Institute, Cerrito 1248, (1010) Buenos Aires, Argentina, AR;(3) Natural Sciences Museum “B. Rivadavia”, Mammals Department, Av. Angel Gallardo 470, (1405) Buenos Aires, Argentina, AR |
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| Abstract: | Milk protein fractions during various stages of lactation in the southern elephant seal Mirounga leonina were analysed. Twelve milk samples were taken from ten females throughout the lactation period during 1990 and 1991 at Stranger
Point, King George Island, South Shetland Islands. Milk samples were subjected to polyacrylamide gel electrophoresis (PAGE).
Samples from different days of lactation gave similar qualitative electrophoretic patterns. True protein content was significantly
higher (P<0.05) at the beginning of lactation, and then remained constant until weaning. Caseins and whey proteins each consisted of
several protein entities (four and five distinct bands respectively). Casein constituted only about 30% of the protein nitrogen,
the remaining 70% being derived from whey proteins. There was some variation in concentration of casein and whey proteins
as a function of time (P<0.0.5).
Received: 28 July 1993/Accepted: 25 July 1995 |
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