Formation of non-spheroidal aggregates by adult rat hepatocytes on polyacrylamide coated surfaces |
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| Authors: | Takayoshi Tokiwa Junko Kano Xian-Yian Meng Raman Bahulekar Makoto Kodama |
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| Institution: | (1) Bionic Design Group, National Institute for Advanced Interdisciplinary Research, 1-1-4 Higashi, Tsukuba, 305 Ibaraki, Japan |
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| Abstract: | Summary Adult rat hepatocytes in primary culture formed floating, non-spheroidal aggregates on the surfaces coated with 1 % polyacrylamide. In the cells of aggregates, DNA synthetic capacity was limited, but albumin secreting capacity was maintained at least for 2 weeks. These properties were influenced by addition of serum. |
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