Inheritance of marker and target genes in seed and vegetative progenies of transgenic tobacco plants carrying the buckwheat serine protease inhibitor gene |
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Authors: | N V Khadeeva E Yu Yakovleva |
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Institution: | 1.Vavilov Institute of General Genetics,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | The buckwheat serine protease inhibitor (BWI-1) target gene (ISP) was expressed under the control of the constitutive 35S promoter of the cauliflower mosaic virus was expressed in transgenic
tobacco plants and conferred antibacterial resistance. A stable and linked inheritance and expression of the marker nptII and target genes were observed in a random sample of independent transgenic tobacco plants after longterm propagation by
nodal segments or multiple (for 1.5 years) regenerations under nonselective conditions; the transgene insert was preserved
in the T1 seed progeny. Transgenic plants displayed numerous alterations in microsporogenesis. A loss of kanamycin (Km) resistance
was accompanied by a loss of antibacterial activity in two lines. Segregation was observed for Km resistance in line C7 and
for seedling size in line C22. |
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