Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase |
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| Authors: | Laura Pastorino Elena Dellacasa Mohamed R. Noor Tewfik Soulimane Paolo Bianchini Francesca D'Autilia Alexei Antipov Alberto Diaspro Syed A. M. Tofail Carmelina Ruggiero |
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| Affiliation: | 1. Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Genova, Italy.; 2. Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland.; 3. Nanophysics, Istituto Italiano di Tecnologia, Genova, Italy.; 4. PlasmaChem GmbH, Berlin, Germany.; 5. Materials and Surface Science Institute, University of Limerick, Limerick, Ireland.; Michigan State University, United States of America, |
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| Abstract: | Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacting with cytochrome c oxidase were permeable and that the permeability characteristics of the microcapsule shell depend on the shell components. This work provides a significant input towards the fabrication of an integrated device made of biological components and based on specific biomolecular functions and properties. |
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