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NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins
Authors:Xuejun Yao  Ulrich H N Dürr  Zrinka Gattin  Yvonne Laukat  Rhagavendran L Narayanan  Ann-Kathrin Brückner  Chris Meisinger  Adam Lange  Stefan Becker  Markus Zweckstetter
Abstract:Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-state NMR spectroscopy. We show that the method allows analysis of the solvent protection of single residues in liposome-embedded proteins such as the 349-residue Tom40, the major protein translocation pore in the outer mitochondrial membrane, which has resisted structural analysis for many years.
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