Effects of divalent cations on phosphatase secretion in cultured tobacco cells |
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Authors: | Katsuji Ueki |
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Institution: | Laboratory of Applied Microbiology, Faculty of Agriculture, Yamagata University, Tsuruoka, Yamagata 997, Japan |
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Abstract: | Some differences were found between Mg2+- and Ca2+-stimulated phosphatase secretion in cultured tobacco cells. The effect of Mg2+ ions was greater than that of Ca2+ ions, and Ca2+ ions at below 1 mM rather depressed the secretion. Upon the addition of Mg2+ ions plus Ca2+ ions, a synergistic stimulation of the secretion occurred. Different influences on the effects of Mg2+ and Ca2+ ions on the secretion were exerted by treating cells with metabolic inhibitors that reduced the level of cellular metabolic energy. Phosphate (Pi) and arsenate did not depress the secretion in the presence of Mg2+ ions, but did depress it in the presence of Ca2+ ions. These results strongly suggested that the secretion of phosphatase involved at least two different steps affected by divalent cations. |
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Keywords: | Solanaceae cultured tobacco cells enzyme secretion phosphatase divalent cations |
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