Ovarian and plasma PGE and PGF levels in naturally ovulating brook trout (Salvelinus fontinalis) and the effects of indomethacin on prostaglandin levels

Plasma and ovarian levels of PGE and PGF were measured by radioimmunoassay in naturally ovulating brook trout ($\text{Salvelinus fontinalis}$). At 12 hours post-ovulation, PGF levels in plasma and ovarian tissue were significantly elevated over levels in gravid controls. Plasma PGF levels were still elevated 5 – 7 days post-ovulation. Indomethacin treatment (10 ug/gm body weight) of ovulatory brook trout significantly decreased plasma and ovarian PGF levels by 12 hours post-ovulation. No significant differences were observed in plasma PGE levels, through gravid controls contained significantly elevated ovarian PGE levels as compared to ovulated fish.