Detection of leukotriene C4 and D4 in the exudate of rat carrageenin-induced pleurisy |
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Authors: | Akinori Ueno Kunio Tanaka Makoto Katori |
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Institution: | Department of Pharmacology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228, Japan |
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Abstract: | Rat carrageenin-induced pleurisy was used as an acute exudative inflammatory model. The crude ethanol extract of the pleural fluid at 5 hr after carrageenin injection caused the very slow contraction of guinea-pig ileum, which was antagonized by FPL 55712 (1 μg/ml). The ethanol extract was cleaned by LH-20 and was rendered for separation of LTC4 and LTD4 by reversed-phase high-performance liquid chromatography (HPLC). Two peaks which showed the same retention time on HPLC as those of LTC4 and LTD4 had the contractile activity of guinea-pig ileum and the ratios of the contractile activity to the height on HPLC agreed with those of synthetic LTC4 and LTD4. Two peaks of Δ6-trans-LTB4, 5S,12R-(E,E,E,Z)-diHETE and 5S, 12S-(E,E,E,Z)-diHETE, were detected, but the appreciable amount of LTB4 was smaller than that of each Δ6-trans-LTB4 in the pleural fluid at 5 hr. |
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