Banding patterns of Chinese hamster chromosomes revealed by new techniques

Two kinds of techniques were newly developed to reveal banding patterns of the Chinese hamster chromosomes. Both techniques were essentially the same as those used for the extraction of proteins, and one of them could induce bands in chromosome arms in only a few seconds. Banding patterns produced by these techniques appeared to be identical to those induced by the methods reported by previous workers, requiring post-fixation incubation of slides in a warm saline. —The banding pattern was typical for each chromosome pair, permitting unequivocal identification of several pairs which were hardly distinguished by the conventional staining procedures. It was confirmed that these patterns had been well preserved in the chromosomes of a cultured cell line.Contribution No. 870 from the National Institute of Genetics, Japan. This work was supported in part by a grant (No. 9001) from the Ministry of Education in Japan.