Production of IGF-II-related peptide by an anaplastic cells line (AT-3) established from the dunning prostatic carcinoma of rats |
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| Authors: | Yuhsi Matuo Nozomu Nishi Hideyuki Tanaka Ikuharu Sasaki John T Issacs Fumio Wada |
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| Institution: | (1) Department of Endocrinology, Kagawa Medical School, 1750, Miki-cho, Kita-gun, 761-07 Kagawa, Japan;(2) Amano Pharmaceutical Co. Ltd, 51, Nishiharu-cho, Nishikasugai-gui, 481 Aichi, Japan;(3) Department of Urology and Oncology Center, School of Medicine, Johns Hopkins University, 21205 Baltimore, Maryland |
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| Abstract: | Summary AT-3 cells, one of anaplastic cell lines established from the Dunning prostatic carcinoma of rats, were able to grow under
serum-free conditions in a state of suspension detached from a substratum. Radioimmunoassays using monoclonal antibody against
rat insulin-like growth factor II (IGF-II) revealed the presence of IGF-II-related peptide in acid-ethanol extracts extracsts
of lyophilized serum-free media conditioned by AT-3 cell. The peptide contents in the culture media increased with increase
in cell number; 71 ng at 3.0 × 106 cells and 449 ng at 4.6 × 107 cells. IGF-II-related peptide was hardly detectable in acid-ethanol extracts of AT-3 cells harvested after 13-days culture.
These results indicate that AT-3 cells produce IGF-II-related peptide ana may release it into the culture media.
Editor's statement One or more members of the insulin-like growth factor family have been established previously as mitogen
for isolated prostate cells. This report suggests that IGF-II member of the family may be involved in autocrine support of
cells from highly malignant prostate tumors. |
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| Keywords: | growth factors IGF-II prostate cancer cell culture |
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