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约氏疟原虫配子体形成的形态学特征
引用本文:毛映红,徐蓓,刘宝丰. 约氏疟原虫配子体形成的形态学特征[J]. 动物学杂志, 2004, 39(3): 72-75
作者姓名:毛映红  徐蓓  刘宝丰
作者单位:中国医学科学院,中国协和医科大学基础医学研究所,北京,100005;中国医学科学院,中国协和医科大学基础医学研究所,北京,100005;中国医学科学院,中国协和医科大学基础医学研究所,北京,100005
基金项目:国家自然科学基金 (No.386 0 2 4 9),世界银行,国立新加坡大学的部分实验资助
摘    要:应用六氰酸铁钾和锇酸双染色法的透射电镜技术,对红内期疟原虫配子体形成过程中的形态生物学特征进行识别。结果表明,配子体是一渐进发育的单核、惰性(团块样、虫体不活跃)虫体,其核旁有一个由扁平囊和泡状小体极性排列组成的细胞器——高尔基体(Golgi complex),虫体被膜下有嗜锇小体。其雌性配子体的结构特点是核质较致密、内质网丰富和被膜下嗜锇小体多;而雄性配子体却是核质较疏松、线粒体发达、内织网和嗜锇小体少。结论是红内期疟原虫配子体形成与裂体增殖过程中形态结构的明显差别,雌雄配子体在结构与发育上明显的差异,都具有特定的生物学与生态学意义。

关 键 词:疟原虫  配子体生成  高尔基体  电镜
文章编号:0250-3263(2004)03-72-04

The Morphological Characteristics of Plasmodium yoelii Gametocytogenesis
MAO Ying Hong XU Bei LIU Bao Feng. The Morphological Characteristics of Plasmodium yoelii Gametocytogenesis[J]. Chinese Journal of Zoology, 2004, 39(3): 72-75
Authors:MAO Ying Hong XU Bei LIU Bao Feng
Abstract:Using the potassium ferricyanide (K 3[Fe(CN) 6]) and osmic acid OsO 4 double dye techniques in TEM microscopy, the morphological characteristics of the gametocytogenesis of Plasmodium yoelii was identified. The gametocytes were shown to be gradually developed mononuclear and inactive (clot like) bodies. There is a polarized organelle, Golgi complex consisted of several saccules and some vesicles beside the nucleus and there are some osmiophilic bodies beneath the parasite pellicule.The characteristics of female gametocytes includeis dense nucleoplasma, rich endoplasmic reticulum and more osmiophilic bodies, while the male gametocytes are characterized by a less densified nucleoplasma, fully developed michtochondira and fewer osmiophilic bodies. These differences between gametocytogenesis and schizogony as well as the structure and development of the female and male gametocytes there are importantly ecologic significances.
Keywords:Plasmodium yoelii  Gametocytogenesis  Golgi complex  E.M microcopy
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