Induction of toll-like receptor 2 positive antigen-presenting cells in spleen of pristane-induced arthritis in rats |
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Authors: | Wenhua Zhu Liesu Meng Congshan Jiang Weikun Hou Jing Xu Bo Wang Shemin Lu |
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Institution: | (1) Department of Genetics and Molecular Biology, Xi’an Jiaotong University School of Medicine, Western Yanta Road 76, Xi’an, P.O. Box 81, 710061, Shaanxi, China;(2) Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi’an, 710061, Shaanxi, China;(3) Department of Epidemiology and Health Statistics, Xi’an Jiaotong University School of Medicine, Xi’an, 710061, Shaanxi, China; |
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Abstract: | Toll-like receptors (TLRs) have been found to contribute to the pathogenesis of rheumatoid arthritis (RA). The aim of this
study is to investigate the regulation and potential role of TLR2 in spleen of pristane-induced arthritis (PIA) rat, which
can be used to further understand the mechanisms of RA. Arthritis in DA rats was induced by pristane. TLR2 expression in spleen
was detected by real-time quantitative PCR and western blotting, and TLR2 expression at both mRNA and protein levels was upregulated
in PIA rats. Peptidoglycan (PGN) was systemically administrated to PIA rats, and arthritis severity was evaluated macroscopically
and microscopically. Results showed that systemic administration of PGN to PIA rats obviously deteriorated arthritis severity.
TLR2 expression on splenocytes and different types of immune cells was measured by flow cytometry. And it was found that TLR2
was mainly expressed on antigen-presenting cells (APCs) of spleen, and the proportion of TLR2+ dendritic cells and macrophages in spleen of PIA rats was increased remarkably. Thus, we conclude that the induction of TLR2+ APCs in spleen may participate in the maintenance of PIA. |
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