Different genetic patterns in avian Toll-like receptor (TLR)5 genes |
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Authors: | Wenke Ruan Yanhua Wu Shijun J Zheng |
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Institution: | (1) College of Animal Science and Technology, Beijing University of Agriculture, Beijing, 102206, China;(2) Department of Laboratory Animal Science, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China;(3) State Key Laboratory of Agrobiotechnology and Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, 2 Yuan-Ming-Yuan West Road, Beijing, 100193, China; |
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Abstract: | Toll-like receptors (TLRs) mediate immune response via recognition of pathogen-associated molecular patterns (PAMPs), thus
play important roles in host defense. Polymorphisms of TLR5 may affect their recognition of bacterial flagellin, leading to
varied host resistance to pathogenic infections. Here, we cloned TLR5 genes from Common Pheasant, Guinea fowl and 9 Chicken
breeds and analyzed their sequences. The open reading frames of TLR5 were sequenced. Amino acid analysis indicated that TLR5
from Chicken breeds shared 99.4–99.9% homology. The amino acid homology of TLR5 ranged from 92.1 to 92.5% between Chickens
and Guinea fowl, 95.7–96.1% between Chickens and Turkey, 94.3–94.7% between Chickens and Common Pheasant, and 79.9–80.1% between
Chickens and Zebra-finch. Different genetic patterns were determined among Chickens, Common Pheasant, Guinea fowl, Turkey
and Zebra-finch. It was found that there were 92 amino acid polymorphic sites, among which 5 sites in chicken TLR5, 63 sites
in Guinea fowl TLR5 and 44 sites in Common Pheasant TLR5. Our data indicate that the positive Darwinian selection occurred
in avian TLR5 genes since frequency of non-synonymous (d
N
) > frequency of synonymous (d
S
). These results also demonstrate that avian TLR5 genes are polymorphic among avian breeds, suggesting a varied resistance
among breeds of avian. This information might be of help to improve the health of avian by breeding and vaccination. |
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