Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis |
| |
Authors: | Richard J Wheeler Keith Gull and Eva Gluenz |
| |
Institution: | (1) The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK |
| |
Abstract: | Background Many trypanosomatid protozoa are important human or animal pathogens. The well defined morphology and precisely choreographed
division of trypanosomatid cells makes morphological analysis a powerful tool for analyzing the effect of mutations, chemical
insults and changes between lifecycle stages. High-throughput image analysis of micrographs has the potential to accelerate
collection of quantitative morphological data. Trypanosomatid cells have two large DNA-containing organelles, the kinetoplast
(mitochondrial DNA) and nucleus, which provide useful markers for morphometric analysis; however they need to be accurately
identified and often lie in close proximity. This presents a technical challenge. Accurate identification and quantitation
of the DNA content of these organelles is a central requirement of any automated analysis method. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|