Structure identification and fermentation characteristics of pinoresinol diglucoside produced by <Emphasis Type="Italic">Phomopsis</Emphasis> sp. isolated from <Emphasis Type="Italic">Eucommia ulmoides</Emphasis> Oliv |
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Authors: | Email author" target="_blank">Junling?ShiEmail author Chao?Liu Laping?Liu Baowei?Yang Yinzhi?Zhang |
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Institution: | (1) College of Food Science and Engineering, Northwest A & F University, 28 Xinong Road, Yangling, Shaanxi, 712100, China;(2) State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China |
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Abstract: | Pinoresinol diglucoside (PDG) is the important antihypertensive compound in Eucommia ulmoides Oliv., a traditional Chinese herb medicine. The research objective was to certify the possibility of producing PDG through
fermentation. PDG-producing endophytic fungi were isolated from E. ulmoides Oliv., and the highest PDG-yielding (11.65 mg/L) isolate, XP-8, was identified as Phomopsis sp. according to the morphological characteristics and the phylogenetic tree constructed on the basis of the gene sequence
in the internal transcribed spacers district. The microbial PDG was isolated by using S-8 resin and purified to a purity of
98.7% using preparative high-performance liquid chromatography (HPLC). Information obtained from the UV spectrum (277 and
227 nm, in water solution), infra-red spectrum (3,428; 2,930; 2,877; 1,637; 1,600; and 1,513; 1,460; 1,421; 1,269; 1,223;
1,075; 658 cm−1, in powder), molecular weight (682 Da, measured using HPLC-electrospray ionization mass spectrometry (ESI/MS) and tandem
mass spectrometry), and nuclear magnetic resonance analysis show the microbial PDG is (+)-1-pinoresinol 4,4′-di-O-β-d-glucopyranoside, same as the plant-derived PDG. The microbial PDG is stable in pH range from 3 to 11 but less stable at temperature
higher than 90 °C and in light exposure. During the fermentation, PDG production outside cells starts at the later stage of
cell growth when the residual sugar in the medium was low. The study reveals the possibility for production of PDG by fermentation. |
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