Production of polyhydroxyalkanoates by <Emphasis Type="Italic">Burkholderia cepacia</Emphasis> ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate |
| |
Authors: | Wenyang Pan Joseph A Perrotta Arthur J Stipanovic Christopher T Nomura James P Nakas |
| |
Institution: | (1) Department of Environment and Forest Biology, SUNY-College of Environmental Science and Forestry, Illick 201, 1 Forestry Drive, Syracuse, NY 13210, USA;(2) Department of Chemistry, SUNY-College of Environmental Science and Forestry, Syracuse, NY 13210, USA; |
| |
Abstract: | Sugar maple hemicellulosic hydrolysate containing 71.9 g/l of xylose was used as an inexpensive feedstock to produce polyhydroxyalkanoates
(PHAs) by Burkholderia cepacia ATCC 17759. Several inhibitory compounds present in wood hydrolysate were analyzed for effects on cell growth and PHA production
with strong inhibition observed at concentrations of 1 g/l furfural, 2 g/l vanillin, 7 g/l levulinic acid, and 1 M acetic
acid. Gradual catabolism of lower concentrations of these inhibitors was observed in this study. To increase the fermentability
of wood hydrolysate, several detoxification methods were tested. Overliming combined with low-temperature sterilization resulted
in the highest removal of total inhibitory phenolics (65%). A fed-batch fermentation exhibited maximum PHA production after
96 h (8.72 g PHA/L broth and 51.4% of dry cell weight). Compositional analysis by NMR and physical–chemical characterization
showed that PHA produced from wood hydrolysate was composed of polyhydroxybutyrate (PHB) with a molecular mass (M
N) of 450.8 kDa, a melting temperature (T
m) of 174.4°C, a glass transition temperature (T
g) of 7.31°C, and a decomposition temperature (T
decomp) of 268.6°C. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|