Purification and characterization of soluble human IL-6 receptor expressed in CHO cells |
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| Authors: | K Yasukawa T Saito T Fukunaga Y Sekimori Y Koishihara H Fukui Y Ohsugi T Matsuda H Yawata T Hirano |
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| Institution: | Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa. |
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| Abstract: | An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensitivity of M1 cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml. |
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