Antimicrobial resistance and toxin gene profiles of Staphylococcus aureus strains from Holstein milk |
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| Authors: | Y. Wang G. Guo T. Usman D. Hao X. Tang Y. Zhang Y. Yu |
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| Affiliation: | 1. Key Laboratory of Agricultural Animal Genetics and Breeding, Ministry of Agricultural, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, , Beijing, China;2. National Center for Molecular Genetics and Breeding of Animal, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, , Beijing, China;3. Beijing Sanyuan Lvhe Dairy Cattle Center, Beijing Sanyuan Breeding Technology Co. Ltd., Capital Agribusiness Group, , Beijing, China;4. College of Food Science and Engineering, Northwest A & F University, , Yangling, China |
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| Abstract: | Isolation of Staphylococcus aureus (Staph. aureus) from Holstein milk samples with mastitis and nonmastitis was conducted to estimate its prevalence, antimicrobial resistance and toxin genes. A total of 353 milk samples were collected from three Chinese Holstein herds. Fifty‐three Staph. aureus isolates collected from 29 Staph. aureus‐positive samples were characterized via antimicrobial susceptibility, toxin genes and Pulsed‐field Gel Electrophoresis (PFGE) profiles. The prevalence of Staph. aureus was 4·0–9·5% in mastitic and 7·3–11·5% in nonmastitic samples in the analysed herds. Approximately 61·0% of Staph. aureus strains isolated from mastitis cows were resistant to ≥10 antimicrobials compared with 0% of isolates with nonmastitis. The most frequently observed super antigenic toxin gene was pvl (41·5%) followed by seh + pvl (13·2%). We did not find mecA‐positive methicillin‐resistant Staph. aureus (MRSA) strains, while mecA‐negative MRSA strains were identified in the three herds. PFGE results suggested potential transmission of Staph. aureus strains in different farms. These results open new insights into Staph. aureus transmission and antimicrobial resistance of Holstein dairy cows and into developing strategies for udder health improvement of dairy cattle. |
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| Keywords: | antimicrobial resistance Holstein mastitis
PFGE
Staphylococcus aureus
toxin genes |
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