Sensitivity of Zymoseptoria tritici Isolates from Tunisia to Pyraclostrobin,Fluxapyroxad, Epoxiconazole,Metconazole, Prochloraz and Tebuconazole |
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Authors: | Karima Taher Sarah Graf Mohamed Moez Fakhfakh Halim B H Salah Amor Yahyaoui Salah Rezgui Bouzid Nasraoui Gerd Stammler |
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Institution: | 1. Institut National des Grandes Cultures, , BP. 120, 8170 Bou‐Salem, Tunisia;2. BASF SE, Agricultural Center, Fungicide Resistance Research, , D‐67117 Limburgerhof, Germany;3. International Maize and Wheat Improvement Center (CIMMYT) Km. 45, Carretera México‐Veracruz El Batán, , Texcoco, Mexico;4. Institut National Agronomique de Tunis, Université de Carthage, , 1002 Tunis, Tunisia |
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Abstract: | Sensitivity of 159 isolates of Zymoseptoria tritici collected from durum wheat fields in Tunisia in 2012 was analysed towards pyraclostrobin, fluxapyroxad, epoxiconazole, metconazole, prochloraz and tebuconazole using microtiter tests. All isolates were found to be highly sensitive to pyraclostrobin with EC50 <0.01 mg/l with the exception of three isolates from the same field with higher EC50 values (>0.5 mg/l). These three isolates carried a mutation in the cytochrome b gene encoding the G143A substitution. This is the first report of quinone outside inhibitors (QoI) resistance in Z. tritici in Tunisia. Sensitivity towards r fluxapyroxad was in a narrow range with EC50 values ranging between 0.013 and 0.125 mg/l, which can serve as baseline sensitivity data for the future. Demethylation inhibitors sensitivity varied across a broad range with the data indicating a slight shift in sensitivity when compared to a previous study on the 2010 population. No highly sensitive strains were isolated from samples from fields, which had received three or four DMI applications. |
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Keywords: | demethylation inhibitors quinone outside inhibitors succinate dehydrogenase inhibitors sensitivity septoria leaf blotch |
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