Occurrence of Ralstonia solanacearum Race 2 Biovar 1 Associated with Moko Disease of Banana (Musa paradisiaca cv. Nipah) in Malaysia |
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| Authors: | Dzarifah Zulperi Kamaruzaman Sijam Zainal A Mior Ahmad Yahya Awang Tavga Sulaiman Rashid |
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| Affiliation: | 1. Department of Plant Protection, Faculty of Agriculture, University Putra Malaysia, UPM, , Serdang, Selangor Darul Ehsan, 43400 Malaysia;2. Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, UPM, , Serdang, Selangor Darul Ehsan, 43400 Malaysia |
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| Abstract: | During June 2011 to March 2012, Moko disease symptoms were observed in banana cv. Nipah in two Malaysian states. The primer pairs ISRso19F/ISRso19R were used for defined identification of Ralstonia solanacearum race 2 strain. PCR amplification of all isolates produced a 1900 amplicon and exhibited 93% phylogenetic similarity with reference strain (AF450275). Based on symptoms, biochemical tests, pathogenicity assay, molecular and phylogenetic studies, we concluded that the isolated bacterium was R. solanacearum race 2 biovar 1. |
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| Keywords: | ISRso19 Malaysia Moko disease Musa paradisiaca cv. Nipah Ralstonia solanacearum race 2 biovar 1 |
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