Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis |
| |
| Authors: | Kinoshita Kazuhisa Noetzel Tim L Pelletier Laurence Mechtler Karl Drechsel David N Schwager Anne Lee Mike Raff Jordan W Hyman Anthony A |
| |
| Institution: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany. kinoshita@mpi-cbg.de |
| |
| Abstract: | Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
