Labeling of Crithidia fasciculata DNA with [3H]Thymidine |
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| Authors: | AGDA M SIMPSON LARRY SIMPSON |
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| Institution: | Department of Biology, University of California, Los Angeles, California 90024 |
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| Abstract: | Attempts at continuous labeling of Crithidia fasciculata DNA with 3H]thymidine led to a pulse-chase situation due to a cell-mediated conversion of thymidine to thymine in the medium. The uptake of thymine was slow compared to that of thymidine. Neither the addition of deoxyadenosine nor the sequential addition of several aliquots of 3H]thymidine had an effect on the pattern of labeling. |
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| Keywords: | Crithidia fasciculata thymidine DNA thymidine phosphorylase |
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