Proposed pathway for biosynthesis of the S-layer glycoprotein of Bacillus alvei. |
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Authors: | E Hartmann P Messner G Allmeier and H Knig |
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Institution: | E Hartmann, P Messner, G Allmeier, and H König |
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Abstract: | The outer surface of the murein sacculus of the eubacterium Bacillus alvei is covered by a surface layer (S-layer) glycoprotein. The glycan chain of this S-layer glycoprotein consists of trisaccharide repeating units with ManNAc, Gal, and Glc as constituents. From cell extracts of B. alvei, nucleotide-activated derivatives of ManNAc, Gal, Glc, and GlcNAc were isolated. Furthermore, GDP- and dolichyl-activated oligosaccharides were obtained. On the basis of the isolated putative glycoprotein precursors, a pathway for the biosynthesis of the oligosaccharide chain is proposed. |
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