Phenotypic and genetic variations in crown-gall tumour cells of tobacco |
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Authors: | A. Mouras I. Negrutiu Y. Dessaux |
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Affiliation: | (1) Avenue des Facultés, Laboratoire Biologie Cellulaire, F-33405 Talence, France;(2) Instituut voor Moleculaire Biologie, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium;(3) Faculté des Sciences, GRIMP-INRA Institut de Microbiologie, F-91405 Orsay, France |
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Abstract: | Summary Phenotypic and genetic variations of tumour cells were analysed both quantitatively and qualitatively in clones and subclones of a crown-gall strain. Thus, growth rates, grafting tests, octopine synthesis, estimations of the T-DNA contents, modifications in the numbers, and structures of chromosomes were examined. Phenotypic variations are closely associated with genetic changes, including variation in chromosome number (which is shown to be non-specific to the tumoral state) and, above all, variation in the copy-number of T-DNA sequences per cell, and structural rearrangements of chromosomes. Such rearrangements are characterized by specific marker chromosomes in the tumour cells and they correlate with the degree of oncogenicity of the cells. |
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Keywords: | Phenotypic/genetic variations Tumour crown gall cells Octopine T-DNA Chromosome markers |
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