Yeast vitality determination based on intracellular NAD(P)H fluorescence measurement during aerobic-anaerobic transition |
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| Authors: | M. Kuřec G. Kuncová T. Brányik |
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| Affiliation: | (1) Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, 166 28 Prague, Czech Republic;(2) Institute of Chemical Process Fundamentals, Academy of Sciences, 165 02 Prague, Czech Republic |
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| Abstract: | This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied. |
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