Requirement of the newly recognized ilvJ gene for the expression of a valine transaminase activity in E. coli K-12 |
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Authors: | John Guardiola |
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Institution: | (1) International Institute of Genetics and Biophysics, C.N.R., Via G. Marconi 10, I-80125 Naples, Italy |
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Abstract: | Summary The isoleucine- -ketoglutarate and valine- -ketoglutarate transaminase activities have been attributed to an enzyme coded in E. coli K-12 by the ilvE gene. I report here evidence that these two activities can be dissociated and appear to be the products of two different genes. Mutants altered in the ilvE gene are devoid of isoleucine- -ketoglutarate transaminase activity and possess a normal valine- -ketoglutarate transaminase activity. I describe here mutants lacking valine transaminase activity. They are altered in a gene, ilvJ, located between ilvE and ilvD at 83 min on the E. coli K-12 map. Temperature-sensitive revertants of the mutant containing the ilvJ mutation show a temperature-sensitive valine- -ketoglutarate transaminase activity. I conclude that ilvJ is the structural gene for valine- -ketoglutarate transaminase. |
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