Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris |
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Authors: | R Ramon JX Feliu O Cos JL Montesinos FX Berthet F Valero |
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Institution: | Departament d'Enginyeria Química ETSE, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. |
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Abstract: | The Pichia pastoris expression system is widely used for the production of recombinant proteins. A simple and efficient experimental set-up allowing on-line monitoring of the methanol concentration during the fermentation of P. pastoris based on the detection of the methanol vapor concentration in the exhaust air from fermenter by a tin dioxide (SnO2) semiconductor sensor is described. An experimental procedure to allow precise calibration of the system and to reduce methanol sensor's interferences (>95% reduction) are also presented and discussed. Accuracy and measurement error were estimated about 0.05 g x l(-1) and 6%, respectively. The efficient monitoring of methanol will help to advanced control of recombinant protein production and process optimization. |
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Keywords: | fermentation monitoring on-line methanol Pichia pastoris |
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