Alterations in phenylpropanoid content in soybean roots during low temperature acclimation |
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| Institution: | 1. Plant Growth Regulation Laboratory, University of Lodz, Banacha 12/16, 90-237 Łódź, Poland;2. Institute of Experimental Botany, Laboratory of Phenolic Substances, Academy of Sciences, Rozvojova 135, 165 02 Praha 6, Czech Republic;1. Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas, Av. General Rodrigo Octavio Jordão Ramos, 3000, University Campus, Japiim, CEP: 69077-000, Manaus, AM, Brazil;2. National Institute for Amazonian Research, Av. Efigênio Sales, 2239, 69060-001, Manaus, Amazonas, Brazil;1. Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi, Delhi 110007, India;2. Institute of Molecular Cell Biology, University of Bonn, Bonn, Germany;1. Clermont Université, Université Blaise Pascal, UMR547 PIAF, F-63000 Clermont-Ferrand, France;2. INRA, UMR547 PIAF, 63100 Clermont-Ferrand, France;3. Irstea, Research Unit on Forest Ecosystems (EFNO), Domaine des Barres, 45290 Nogent-sur-Vernisson, France;1. Australian Reseach Council (ARC) Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley 6009, Australia;1. Technical Chemistry, Department of Chemistry, Chemical-Biological Centre, Umeå University, SE-901 87 Umeå, Sweden;2. School of Engineering, University of Borås, SE-501 90 Borås, Sweden;3. Industrial Chemistry and Reaction Engineering, Process Chemistry Centre, Department of Chemical Engineering, Åbo Akademi University, FI-20500 Åbo-Turku, Finland;4. Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden |
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| Abstract: | L-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity, growth and phenolic acid content during low temperature acclimation in soybean (Glycine max. (L.) Merr.) roots were investigated. Elongation of soybean roots was inhibited after the transfer of 3-d-old seedlings grown at 25 to 10 °C. Extractable PAL activity as well as the total amount of phenolics increased 24 h after plant transfer to low temperature. The high pressure liquid chromatography analyses revealed the presence of six phenolic acids in soybean roots: p-hydroxybenzoic, vanillic, syringic, anisic, p-coumaric and ferulic. Analyses of different fractions of phenolic acids showed that during 24 h of low temperature exposure, an increase in the relative level of ester-bound-soluble phenolic acids occurred. The highest increase in this fraction was observed for ferulic acid (26 %). At the same time, a decrease in phenolic glycosides took place. The amount of phenolic acids released after alkaline treatment of the cell wall material was strongly inhibited (3-fold), which may suggest an alteration of the physical properties of the wall in acclimation to low temperature. The possible role of phenolics in acclimation to low temperature in roots is discussed. |
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