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Genomic Sequence Analysis of Bombyx mori Nucleopolyhedrovirus Isolated from Yunnan Sericulture Region,China
Authors:Yonghong Zhang  Feng Zhu  Yulan Shao  Zhenguo Su  Jing Ni  Xingrong Bai
Institution:1.Sericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Science, Mengzi, Yunnan China ;2.College of Life Sciences, Zaozhuang University, Zaozhuang, China
Abstract:The blood pathogens of grasserie caused by Bombyx mori nucleopolyhedrovirus BmNPV have a serious impact on the sericulture industry. To understand the genetic status of BmNPV endemic strains in the Yunnan sericulture region, the structure and complete genome sequence of BmNPV isolated from Baoshan city of Yunnan Province were described and compared to known strains. The BmNPV-Baoshan isolate was a nucleopolyhedrovirus parasitized in silkworm larvae. Its genome has 128, 452 bp with a G + C content of 40.4%. Phylogenetic analysis clustered the virus with China BmNPV isolates; BmNPV-Baoshan was closely related to BomaNPV-S1 (both strains originated from the same ancestor). BmNPV-Baoshan strain has bro-b gene deletion, hr1 missing 4 repeat units of 30-bp palindrome structure compared to BmNPV-T3 strain. The aim of this study was to elucidate the evolution of the virus further and provide insights for the protection of virus-induced hematologic sepsis.
Keywords:Bombyx mori  Complete genome  Baculovirus  BmNPV isolate  Baoshan isolate
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