Biosynthesis of triacylglycerol in Thunbergia alata: Additional evidence for involvement of phosphatidylcholine in unusual monoenoic oil production |
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| Institution: | 1. CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei, Anhui 230027, China;2. State Key Laboratory of Fire Science, University of Science and Technology of China, Hefei, Anhui 230026, China;3. PLA Army Academy of Artillery and Air Defense, Hefei, Anhui 230031, China;3. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843;4. Departments of Medicine and Pharmacology, University of California, San Diego, La Jolla, California 92093;5. Department of Molecular and Biomedical Sciences, Jo?ef Stefan Institute, Jamova 39, Ljubljana, Slovenia;6. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ve?na pot 113, 1000 Ljubljana, Slovenia;1. Department of Ophthalmology, Kanazawa Medical University, Uchinada, Japan;2. Department of Ophthalmology, Visual Science, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan;1. Disease Control and Prevention Center, National Center for Global Health and Medicine Hospital, Tokyo, Japan;2. Emerging and Reemerging Infectious Diseases, Graduate School of Medicine, Tohoku University, Sendai, Japan;3. AMR Clinical Reference Center, National Center for Global Health and Medicine Hospital, Tokyo, Japan;4. Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium;5. Center for Clinical Sciences, National Center for Global Health and Medicine, Tokyo, Japan;6. AIDS Clinical Center, National Center for Global Health and Medicine Hospital, Tokyo, Japan;7. Division of Respiratory Medicine, National Center for Global Health and Medicine Hospital, Tokyo, Japan |
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| Abstract: | The seed oil of Thunbergia alata has an unusual fatty acid composition which consists of more than 80 % 16:1Δ6. This fatty acid is produced in the plastid by the action of a Δ6 palmitoyl (16:0)-ACP desaturase. To examine the biosynthesis of triacylglycerol (TAG) containing high concentrations of this unusual monoenoic fatty acid, endosperm dissected from developing T. alata seeds was labeled with 1-14C]-acetate. At early time points (5–15 min), the predominant labeled lipid was PC whereas at later time points (greater than 30 min) TAG became the major labeled lipid. Analysis of the acyl group composition of each lipid revealed that radiolabeled 16:1Δ6 was highest at early time points in PC while at later time points, it was found to be highest in TAG. Further analysis of the distribution of labeled acyl groups within PC indicated that 16:1Δ6 at the sn-2 position comprised the majority (55–78 %) of total labeled acyl groups whereas 16:1Δ6 at the sn-1 position constituted only a small fraction (12–15 %) of the total labeled acyl groups. In contrast, unlabeled PC contained lower amounts of 16:1Δ6 (16 %) at the sn-2 position. These results are consistent with previous studies suggesting a flux of novel monoenoic acids through PC during TAG biosynthesis, and furthermore imply a stereospecific flux through the sn-2 position of PC. |
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