An improved colony hybridization method with significantly increased sensitivity for detection of single genes |
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Authors: | Renata Maas |
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Institution: | Department of Microbiology, New York University School of Medicine, New York, New York 10016 U.S.A. |
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Abstract: | By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sensitivity, probably by increasing the total denatured plasmid DNA fixed to the paper. |
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