Gene expression during fruit ripening in avocado |
| |
Authors: | Rolf E. Christoffersen Erich Warm George G. Laties |
| |
Affiliation: | (1) Department of Biology, University of California, 90024 Los Angeles, CA, USA;(2) Molecular Biology Institute, University of California, 90024 Los Angeles, CA, USA;(3) Present address: Botanisches Institut der Universität, Menzingerstrasse 67, D-8000 München 19, Federal Republic of Germany |
| |
Abstract: | The poly(A) +RNA populations from avocado fruit (Persea americana Mill cv. Hass) at four stages of ripening were isolated by two cycles of oligo-dT-cellulose chromatography and examined by invitro translation, using the rabbit reticulocyte lysate system, followed by two-dimensional gel electrophoresis (isoelectric focusing followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of the resulting translation products. Three mRNAs increased dramatically with the climacteric rise in respiration and ethylene production. The molecular weights of the corresponding translation products from the ripening-related mRNAs are 80,000, 36,000, and 16,500. These results indicate that ripening may be linked to the expression of specific genes. |
| |
Keywords: | Fruit ripening Gene expression mRNA Persea |
本文献已被 SpringerLink 等数据库收录! |
|