由不同细胞核背景引起的小麦细胞质雄性不育系(CMS)叶绿体蛋白质组的变化(简报)

叶绿体和线粒体是高等植物细胞内2种重要的细胞器。由于细胞质雄性不育(CMS)被认为是一种由线粒体基因编码的性状,因此,近10多年来,国内外研究者对线粒体基因组结构与功能、由线粒体基因编码的与CMS相关蛋白的研究积累了大量的资料。与线粒体相比,叶绿体与CMS关系的研究相对滞后。虽然一些研究者在核不育水稻中,观

THE CHANGES IN CHLOROPLAST PROTEOME CAUSED BY DIFFERENT NUCLEAR BACKGROUND IN CYTOPLASMIC MALE-STERILE (CMS) OF WHEAT

Comparative studies of chloroplast proteome on different developmental stage (seeding,tillering,shooting,booting stage)of leaves have been made in isoplamic allonuclear male- sterile lines Nongda 3237A,Xiaoyan No.6A and their maintainer lines by 2D-PAGE.The results indicated that no obvious differences were found in chloroplast proteome between Nongda 3237A, Nongda 3237B,Xiaoyan No.6A,Xiaoyan No.6B at different developmental stages of leaves. Differences were found just at booting stage in Xiaoyan No.6A and its maintainer lines.Obvious differences,however,were observed in chloroplast proteome between isoplasmic allonuclear male- sterile lines Nongda 3237A and Xiaoyan No.6A.For instance,2 protein spots(pI5.4/34kDa,pI5.4/ 32kDa)at seedling stage,6 protein spots(pI5.4/80kDa,pI5.4/65kDa,pIS.4/60kDa,pIS.4/48kDa, pI5.4/40kDa,pIS.4/35kDa)at booting stage were present in Xiaoyan No.6A and absent in Nongda 3237A.pI6.3/18kDa was present at seedling stage in Nongda 3237A and absent in Xiaoyan No. 6A.pI6.8/28kDa protein spot revealed the developmental changes.It was present in leaves at seedling,tillering,shooting stages and absent at flowering stage in Nongda 3237A.No development changes of the protein spot were observed in Xiaoyan No.6A.These experiment results demon- strated that it was possible chloroplast proteome wasn't relative to the cytoplasmic male-sterile characteristics.But nuclear background in male-sterile lines can obviously affect chloroplast protein composition.Distant relative on nuclear-cytoplasmic has larger differences on chloroplast proteome than close relative on nuclear-cytoplasmic in CMS.