兔下颌骨慢性化脓性骨髓炎动物模型的建立

目的 探讨下颌骨慢性化脓性骨髓炎的造模方法.方法 成年新西兰兔22只,于下颌骨体外侧近正中联合处开骨窗注入0.1 mL5％鱼肝油酸钠和0.1 mL5.0×108 CFU/mL金黄色葡萄球菌悬液,术后常规回笼饲养.于6周开始采用以下方法检测:①肉眼大体观察；②双能X线骨密度检测；③CBCT(锥束CT)放射学形态观察;④局部细菌培养；⑤组织病理学评价.结果 所有新西兰兔早期精神状况较差,手术区域肉眼观有瘘管并有脓性分泌物.双能X线骨密度检测发现手术区骨密度(BMD)值变化不具有统计学意义(P＞0.05).CBCT显示骨缺损区边缘模糊,有骨破坏迹象.局部取材细菌培养有金黄色葡萄球菌生长.病理切片显示有不同程度的淋巴细胞、中性粒细胞、桨细胞浸润,死骨形成.结论 采用兔下颌骨体外侧近正中联合处造骨缺损并注射一定量细菌悬液的方法能够获得理想的慢性化脓性骨髓炎模型.

Establishment of a rabbit model of chronic suppurative osteomyelitis of the mandible

Objective To establish a rabbit model of chronic suppurative osteomyelitis of the mandible.Methods Twenty-two healthy New Zealand rabbits were used in this study.0.1 mL 5% sodium morrhuate and 0.1 mL 5.0×108 CFU/mL suspension of Staphylococcus aureus were injected into the bone window in the lateral side of mandible body near the middle joint of the mandible.Clinical,cone beam CT（CBCT）,bacteriological and histopathological examinations of the mandibles were performed at 16 weeks after infection.Results All rabbits showed poor mental health,and fistulae and purulent secretion were seen in the early period after infection.Dual-energy X-ray examination showed no significant changes of the bone mineral density（BMD） in the surgical area（P 〉0.05）.CBCT detected a blurred edge of the bone defects and signs of bone destruction.Staphylococcus aureus growth was detected in the surgical area.Pathological examination showed infiltration of lymphocytes,neutrophils and plasma cells,and sequestration.Conclusions A model of chronic osteomyelitis can be successfully established by injection of sodium morrhuate and Staphylococcus aureus suspension into the bone window in the lateral side of mandible body near to the middle joint in rabbits.