恒河猴五日热巴尔通体的分离和柠檬酸合成酶基因的序列分析

目的初步研究巴尔通体在恒河猴体内的存在情况,并分析其柠檬酸合成酶（gltA）的基因序列,判断巴尔通体的种属。方法 16只来自福建的恒河猴,用血琼脂培养基分离可能存在的巴尔通体。根据NCBI数据库上的巴尔通体gltA的基因序列,设计一对引物,以巴尔通体菌落为模板进行扩增,将获得的序列进行克隆测序。结果从3只恒河猴体内成功分离到了巴尔通体病原,并获得了巴尔通体gltA全长基因序列,测序结果表明该序列与五日热巴尔通体同源性99%。结论福建来源的恒河猴种群携带巴尔通体病原,巴尔通体流行地区可能存在鼠与灵长类动物之间病原体的流行和传播。

Isolation of Bartonella quintana from rhesus monkeys and analysis of the gltA gene sequence

Objective To investigate the presence of Bartonella in rhesus monkeys and to analyze the gltA gene sequence. Methods Bartonella were isolated from 16 rhesus monkeys coming from Fujian province, isolated with Guy＇s culture-medium. According to the reported complete nucleotide sequence of gltA gene in NCBI, one pairs of primers were designed and synthesized. The gltA gene was amplified from the lysate of BartoneUa colony by PCR. Then the bands were cloned and sequenced. Results Bartonella pathogen was successfully isolated from 3 rhesus monkeys. The sequencing of gltA showed that the isolated Bartonella belonged to Bartonella quintana, with a homology of 98%. Conclusion Bartonella pathogen is existed in rhesus mokey coming from Fujian province. The prevalence and spreading of Bartonella infection may happen between mouse and primate in the epidemic area.