雷洛昔酚对大鼠垂体的作用

目的 观察雷洛昔酚是否能诱发出催乳素瘤的动物模型以及对PRL水平的影响,以研究雷洛昔酚对大鼠垂体的作用.方法 雌性Wistar大鼠切除卵巢后,分别在皮下埋植含有雷洛昔酚、雌激素和空白硅胶管,术后8周处死大鼠,检测大鼠体重变化、垂体重量变化、血清催乳素(PRL)水平和垂体组织学变化.结果 雷洛昔酚组与阴性对照组大鼠体重无明显统计学差异,与雌激素组大鼠体重具有统计学差异(P＜0.05)；雷洛昔酚组与阴性对照组大鼠垂体重相比无明显差异,与雌激素组大鼠垂体重相比具有统计学差异(P＜0.05)；雌激素组大鼠血清PRL水平最高,阴性对照组血清PRL水平最低,雷洛昔酚组介于两者之间,分别与雌激素组、对照组相比较差异均具有统计学意义(P＜0.05)；雷洛昔酚组与对照组垂体病理为正常细胞形态,雌激素组垂体病理为PRL瘤表现.结论 雷洛昔酚对大鼠垂体有一定的影响,但不能诱发催乳素瘤.

Effects of raloxifene on the pituitary gland in rats

Objective To explore the effects of raloxifene on pituitary gland in rats.Methods Ovariectomized Wistar female rats were implanted with silastic tubes containing raloxifene,17-β estradiol or blank silastic tube alone,respectively.After 8 weeks,all the rats were weighed and sacrifieced.The pituitary glands were collected and weighed.Serum prolactin level（PRL） was tested by solid phase-sandwich method.Results There was no significant difference between the pituitary weights of the raloxifene and control groups.However,the pituitary weight of the estrogen group was significantly higher than that of raloxifene and control groups.（both P 〈0.05）.The serum PRL level in the estrogen group was significantly higher than that in the raloxifene and control groups（both P〈0.05）.The serum PRL level of the raloxifene group was significantly higher than that of the control group（P〈0.05）.The serum PRL level in the raloxifene group was significantly lower than that of the estrogen group（P〈0.05）.Histological examination showed normal pituitary morphology in the raloxifene and control groups,but characteristic changes of prolactinoma were seen in the pituitary gland of estrogen group.Conclusion Raloxifene has a certain impact on rat pituitary gland,but does not induce prolactinoma.