Quantification of D-amino acids in human urine using GC-MS and HPLC |
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Authors: | H. Brückner S. Haasmann A. Friedrich |
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Affiliation: | (1) Institute of Food Technology, University of Hohenheim, Stuttgart, Federal, Garbenstrasse 25, D-70599 Stuttgart, Germany;(2) State Veterinary Laboratory Stuttgart, Stuttgart, Germany |
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Abstract: | Summary The relative amounts of free D-amino acids (D-AA) in the urine of seven healthy volunteers (age 27 to 49 years) were determined using chiral phase (Chirasil-L-Val) capillary gas chromatography in conjunction with selected ion monitoring mass spectrometry. The absolute amounts of free D-AA were determined by pre-column derivatization of the amino acids witho-phthaldialdehyde andN-isobutyryl-L-cysteine followed by high-performance liquid chromatographic separation and fluorescence detection of the isoindol derivatives formed. The following most abundant D-AA were found (highest and lowest absolute and relative amounts): D-Ser (379.8 — 30.1µMol/L; 56.5 — 19.0%), D-Ala (53.8 — 7.6µMol/L; 19.6 — 5.7%), D-Thr (5.8 — 0.25µMol/L; 3.4 — 1.0%), D-Val (3.7 — 0µMol/L; 4.2 — 0%), and D-Phe (3.5 — 0.35µMol/L; 4.8 — 1.4%). |
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Keywords: | Amino acids Amino acid enantiomers Chiral analysis Chirasil-L-Val Gas chromatography Mass spectrometry Selected ion monitoring D-Serine D-Alanine |
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