Flash reactivation of Tris-inactivated chloroplasts |
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| Authors: | Yamashita Takashi; Inoue Yorinao; Kobayashi Yoshichika; Shibata Kazuo |
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| Institution: | 1Institute of Biological Sciences, University of Tsukuba Ibaraki 300-31, Japan
2Laboratory of Plant Physiology, The Institute of Physical and Chemical Research Wako-shi, Saitama 351, Japan |
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| Abstract: | The light conditions required for reactivation of the oxygen-evolvingsystem in Tris-treated chloroplasts were studied by means ofrepetitive flashes. Inactive Tristreated chloroplasts were washedwith reduced 2,6-dichlorophenolindophenol, suspended in a reactivationmedium containing Mn2++ and Ca2++ ions, then illuminated withflashes. Flashes at dark intervals of 2 sec were most effectivefor reactivation, while those at shorter or longer intervalswere less effective. It was deduced that more than two sequentiallight reactions with dark reactions in between were involvedin the reactivation. (Received December 28, 1977; ) |
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