| 猪瘟病毒野毒株持续感染细胞模型的建立 |
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| 引用本文： | 应松成 王在时 等. 猪瘟病毒野毒株持续感染细胞模型的建立[J]. Virologica Sinica, 2002, 17(1): 77-81 |
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| 作者姓名： | 应松成 王在时 等 |
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| 作者单位： | 武汉大学生命科学学院 湖北武汉430072(应松成，郑从义，屈三甫，张楚瑜)，中国兽药监察所 北京100081(王在时) |
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| 基金项目： | 国家重点基础研究发展规划项目“973”（G1999011904） |
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| 摘 要： | 采用猪瘟病毒野毒珠（ＣＳＦＶ３９）感染猪肾传代细胞系ＰＫ－１５，经连续传７９代，建立了稳定的病毒持续感染细胞模型，获得ＣＳＦＶ３９－ＰＫ１５细胞株。用免疫荧光、ＲＴ－ＰＣＲ检测、透射电镜跟踪观察了ＣＳＦＶ３９－ＰＫ１５细胞株连续传代中病毒在细胞内的存在情况。结果表明第９，２９，７９代细胞仍有猪瘟病毒存在，表现出病毒持续感染的基本特征。这为深入研究猪瘟病毒持续感染机理奠定了基础。
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| 关 键 词： | 猪瘟病毒株CSFV39 持续感染 细胞模型 RT-PCR |
Model Establishment of the Cell Persistently Infected bya Field-isolated Virulent Strain of CSFV |
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| YING Song-cheng,ZHENG Cong-yi,QU San-pu,ZHANG Chu-yu,WANG Zai-shi. Model Establishment of the Cell Persistently Infected bya Field-isolated Virulent Strain of CSFV[J]. 中国病毒学(英文版), 2002, 17(1): 77-81 |
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| Authors: | YING Song-cheng ZHENG Cong-yi QU San-pu ZHANG Chu-yu WANG Zai-shi |
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| Abstract: | A porcine kidney cell line(PK-15) was infected by a fiel d-isolated virulent strain of classical swine fever virus (CSFV39).After the in f ected cells had subcultured 79 passages for near eight months,a steady m odel of the cell persistently infected by virus has been established,hence the C SFV39-PK15 cell line was procured.In this experiment immunofluorescence,RT-PCR assa y and electron microscopy observation were used to identify the basic viral feat ures in the CSFV39-PK15 cell. The results showed the 9th,29th,79th CSFV39-PK1 5 cell passages still harbored CSFV and they had the radical characteristics of v iral persistent infection.This endeavor should be the foundation for the furthe r research of CSFV persistent infection. |
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| Keywords: | Classical swine fever virus(CSFV39) Persistent infection Cell model RT-PCR |
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