Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond |
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| Authors: | Kämpfer Peter Witzenberger Reinhard Denner Ewald B M Busse Hans-Jürgen Neef Alexander |
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| Affiliation: | 1. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Giessen;2. Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universiät, Vienna;3. Institut für Mikrobiologie und Genetik, Universität Wien, Vienna, Austria;1. Department of Biology, Advanced Facility for Avian Research, University of Western Ontario, 1151 Richmond St., London, Ontario, Canada N6A 5B7;2. Abt Associates, 1881 Ninth Street, Boulder, CO 80302, USA;3. Cornell University, Department of Mechanical & Aerospace Engineering, 105 Upson Hall, Ithaca, NY 14853, USA;4. Department of Agriculture, Nutrition and Veterinary Sciences, University of Nevada, 1664 N. Virginia St., Reno, NV 89557, USA;1. Center of Emphasis in Infectious Diseases and Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX, 79905, USA;2. Graduate School of Biomedical Sciences, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA;1. Cardiff University, Queen''s Building, Cardiff CF24 3AA, United Kingdom;2. Faculty of Mechanical Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia;3. UTM Centre for Low Carbon Transport in Cooperation with Imperial College London, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia;1. TUM, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Department für Biowissenschaften, Weihenstephaner Steig 23, 85350 Freising, Germany;2. Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Molecular EXposomics (MEX), Ingolstädter Landstr.1, D-85764 Neuherberg, Germany;3. The State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China;3. Departments of Ocular Microbiology, Madurai, Tamil Nadu, India;1. Intraocular Lens and Cataract Services, Madurai, Tamil Nadu, India;2. Cornea and Refractive Surgery Services, Aravind Eye Hospital and Post Graduate Institute of Ophthalmology, Madurai, Tamil Nadu, India |
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| Abstract: | The taxonomy of two strains W-51T and W-52 isolated from a wastewater treatment plant was investigated in a polyphasic approach. The yellow pigmented gram-negative organism contained a quinone system with mainly ubiquinone Q-10, and the polar lipid profile contained a sphingoglycolipid suggesting that both strains belonged to the the alpha-4 subclass of the Proteobacteria. The polar lipid profile consisted furthermore of phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylcholine and of minor amounts of phosphatidylglycerol and phosphatidylmonomethylethanolamine. Sequencing of the 16S rRNA gene supported the allocation into the genus Novosphingobium, together with the type strains of N. subterraneum, N. aromaticivorans, N. stygium, and N. capsulatum, showing similarities of 97.3%, 97.0%, 95.7% and 96.2%, respectively. This allocation was supported by the polyamine profile, which consisted mainly of spermidine. The analysis of the fatty acids revealed 2-OH 13:0, 2-OH 14:0 and 2-OH 15:0, with 2-OH 15:0 as predominant hydroxylated fatty acid. W-51T and W-52 were almost identical with respect to their phenotypic including the majority of the chemotaxonomic properties, identical in their 16S rRNA sequences, and showed 86% DNA-DNA similarity. Both strains were able to reduce nitrate and on the basis of further physiological features, a clear differentiation from all other Novosphingobium species was possible. The DNA-DNA similarities of W-51T to the type strains of N. subterraneum, N. aromaticivorans, and N. capsulatum were below 56%. For these reasons, it is proposed to create a new species with the name Novosphingobium hassiacum sp. nov. |
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