Differentiation of a human monocyte-like cell line by (2′–5′) oligoisoadenylate |
| |
Authors: | A. Schmidt T. Hattori T. Hoffman |
| |
Affiliation: | Biological Therapeutics Branch, National Cancer Institute, Frederick, MD 21701, USA |
| |
Abstract: | Treatment of a human monocyte-like cell line (U-937) by (2'-5')ApApA, the 5' dephosphorylated product of (2'-5')oligo-isoadenylate [oligo(A)] synthetase, an interferon-induced enzyme, was able to induce differentiation, mimicking the effect of interferon treatment. Treatment of U-937 cells with (2'-5')ApApA resulted in morphologic changes, new (monocyte-associated) membrane antigen expression, and acquisition of the capacity to mediate antibody-dependent cellular cytotoxicity (ADCC). (2'-5')ApA and (3'-5')ApApA were without effect. A myeloid cell line (HL-60) which differentiates in response to other agents, but not to alpha-interferon, was not able to differentiate in response to (2'-5')ApApA, despite the ability of interferon to induce (2'-5')oligo (A) synthetase. |
| |
Keywords: | To whom offprint reports should be sent. Present address: Office of Biologies National Center for Drugs and Biologies Division of Biochemistry and Biophysics 8800 Rockvile Pike Bethesda DM 20205 USA. |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|