Biological dosimetry: the potential use of radiation-induced apoptosis in human T-lymphocytes |
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Authors: | R Menz R Andres B Larsson M Ozsahin K Trott N E A Crompton |
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Institution: | (1) Department of Life Sciences, Paul Scherrer Institute, CH-5232 Villigen-PSI, Switzerland, CH;(2) St. Bartholemew's and the Royal London School of Medicine and Dentistry, University of London, UK, GB |
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Abstract: | An assay for biological dosimetry based on the induction of apoptosis in human T-lymphocytes is described. Radiation-induced
apoptosis was assessed by flow cytometric identification of cells displaying apoptosis-associated DNA condensation. CD4 and
CD8 T-lymphocytes were analysed. They were recognized on the basis of their cell-surface antigens. Four parameters were measured
for both cell types: cell size, granularity, antigen immunofluorescence and DNA content. Apoptosis was quantified as the fraction
of CD4-, or CD8-positive cells with a characteristic reduction of cell size and DNA content. At doses below 1 Gy, levels of
radiation-induced apoptosis increased for up to 5 days after irradiation. Optimal dose discrimination was observed 4 days
after irradiation, at which time the dose-response curves were linear, with a slope of 8% ± 0.5% per 0.1 Gy. In controlled,
dose-response experiments the lowest dose level at which the radiation-induced apoptosis frequency was still significantly
above control was 0.05 Gy. After 5 days post-irradiation incubation, intra- and interdonor variations were measured and found
to be similar; thus, apoptotic levels depend more on the dose than on the donor. The results demonstrate the potential of
this assay as a biological dosimeter.
Received: 27 February 1997 / Accepted in revised form: 14 May 1997 |
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