S-100 protein subunits in bovine oviduct epithelium:In situ distribution and changes during primary cell culture |
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Authors: | I Walter and I Miller |
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Institution: | (1) Institute of Histology and Embryology, Veterinary University Vienna, Josef-Baumann-Gasse 1, A-1210 Wien, Austria;(2) Institute of Medical Chemistry, Veterinary University, Vienna, Austria |
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Abstract: | Summary Cultures of bovine oviduct epithelial cells are widely used in co-culture systems to improve the results ofin vitro fertilization. The aim of the present study was to evaluate the suitability of S-100 protein as a differentiation marker
for bovine oviduct epithelial cellsin vitro. The distribution of S-100α and S-100β was examined immunohistochemically in bovine oviduct epitheliumin situ and in primary cell cultures derived from it. Three segments of the Fallopian tube (isthmus, ampulla and fimbriae) were compared
and analysed during different stages of the oestrus cycle (luteal phase and follicular phase). Ciliated and non-ciliated cells
of the epithelium reacted with anti-S-100α, S-100a(αβ) and S-100β antibodies, except for isthmic non-ciliated cells, which
did not bind anti-S-100β or anti-S-100a(αβ). In addition, basal cells never showed immunoreactivity for S-100. In confluent
monolayers of cultured oviduct epithelial cells, disappearance of reactivity for S-100 paralleled morphological signs of dedifferentiation
(loss of cilia, cytoplasmic vacuolization). Free-floating oviduct epithelial cells, in contrast, retained morphological differentiation
and still expressed S-100 antigen even after seven daysin vitro. The immunohistochemical findings were confirmed by polyacrylamide gel electrophoresis and Western blotting. The results
indicate that the presence of S-100 is closely connected to morphological differentiation and to the specific functional condition
of bovine oviduct epithelial cells. |
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