Quantitative genetic studies of A/B zeins using a new model to test non-allelic interactions |
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Authors: | T P Bogyo J W Paulis J A Bietz J S C Smith |
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Institution: | (1) Washington State University, 99164-3144 Pullman, WA, USA;(2) Food Physical Chemistry Research, National Center for Agricultural Utilization Research, USDA-ARS, 61604-3999 Peoria, IL, USA;(3) Department of Technical Support, Pioneer Hi-Bred International, Inc, 7250 NW 62nd Avenue, 50131 Johnston, IA, USA |
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Abstract: | A genetic model developed by Bogyo et al. (1988) for quantitatively inherited triploid endosperm characters (an extension of the well-known Mather-Jinks model) is not well-suited for estimating epistatic interaction effects because it requires the assumption that, in segregating loci, all alleles positively affecting a particular character are in one of the inbred parental lines. To better explain zein inheritance in maize, a new model was developed not relying on this assumption. This model was tested by quantitative analysis of A/B zeins, the predominant prolamin storage proteins of maize, using reversed phase high-performance liquid chromatography of two inbred lines, their reciprocal F1 crosses, the F2 generation, backcrosses, and reciprocal backcrosses to both parent lines. The model required epistatic components to be included for an excellent fit for most protein peaks.Scientific Paper No. 3 Program in Statistics, Washington State University, Pullman WA 99164. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned |
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Keywords: | Zein Zea mays L Corn Genetic linkages RP-HPLC Proteins Prolamins Inheritance Quantitation Model |
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